LOCUS       V003408                 8596 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003408
VERSION     V003408
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Kanda K, Ishida T, Hirota R, Ono S, Motomura K, Ikeda T, Kitamura K,
            Kuroda A.
  TITLE     Application of a phosphite dehydrogenase gene as a novel dominant
            selection marker for yeasts
  JOURNAL   J. Biotechnol. 182-183, 68-73 (2014)
   PUBMED   24786825
REFERENCE   2  (bases 1 to 8596)
  AUTHORS   Hirota R.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2013) Contact:Ryuichi Hirota Hiroshima University,
            Department of Biotechnology; 1-3-1, Higashi-Hiroshima, Hiroshima
            739-8530, Japan
REFERENCE   3  (bases 1 to 8596)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8596)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Biotechnol. 182-183, 68-73 (2014)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-NOV-2013) Contact:Ryuichi Hirota Hiroshima University,
            Department of Biotechnology; 1-3-1, Higashi-Hiroshima, Hiroshima
            739-8530, Japan"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          3695..3764
                     /mol_type="other DNA"
                     /db_xref="taxon:4932"
                     /organism="Saccharomyces cerevisiae"
     misc_feature    1..6
                     /label="EcoRI recognition site"
                     /note="EcoRI recognition site"
     rep_origin      344..1686
                     /label="2u ori"
                     /note="yeast 2u plasmid origin of replication"
     misc_feature    2241..2246
                     /label="EcoRI recognition site"
                     /note="EcoRI recognition site"
     promoter        2278..2498
                     /label="URA3 promoter"
     CDS             2499..3299
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     misc_feature    3378..3383
                     /label="SmaI recognition site"
                     /note="SmaI recognition site"
     regulatory      3695..3764
                     /label="IPCS promoter"
                     /note="IPCS promoter"
                     /regulatory_class="promoter"
     CDS             3765..4775
                     /codon_start=1
                     /gene="OPTptxD"
                     /product="phosphite dehydrogenase"
                     /label="OPTptxD"
                     /protein_id="BAP74305.1"
                     /translation="MKPKVVLTHWVHPEIIELLSASADVIPNTTRETLPRSEVIARAKD
                     ADALMAFMPDSIDSAFLEECPKLRVIGAALKGYDNFDVNACTRHGVWLTIVPDLLTIPT
                     AELTIGLLLGLTRHMLEGDRQIRSGHFQGWRPTLYGSGLTGKTLGIIGMGAVGRAIAQR
                     LAGFEMNLLYCDPIPLNAEQEKAWHVQRVTLDELLEKCDYVVPMVPMAAETLHLIDATA
                     LAKMKTGSYLINACRGSVVDENAVIAALASGKLAGYAADVFEMEEWIRADRPQAIPKAL
                     LDNTAQTFFTPHLGSAVKEVRLEIERQAAMNIIQALAGEKPMGAINQPYPGVKAA"
     gene            3765..4775
                     /gene="OPTptxD"
                     /label="OPTptxD"
     CDS             complement(5089..5481)
                     /gene="MRP17"
                     /label="Small ribosomal subunit protein bS6m"
                     /note="Small ribosomal subunit protein bS6m from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P28778"
     CDS             6153..6341
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    6446..6586
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6772..7360)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7534..8391)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8392..8496)
                     /label="AmpR promoter"
ORIGIN
        1 gaattctgaa ccagtcctaa aacgagtaaa taggaccggc aattcttcaa gcaataaaca
       61 ggaataccaa ttattaaaag ataacttagt cagatcgtac aataaagctt tgaagaaaaa
      121 tgcgccttat tcaatctttg ctataaaaaa tggcccaaaa tctcacattg gaagacattt
      181 gatgacctca tttctttcaa tgaagggcct aacggagttg actaatgttg tgggaaattg
      241 gagcgataag cgtgcttctg ccgtggccag gacaacgtat actcatcaga taacagcaat
      301 acctgatcac tacttcgcac tagtttctcg gtactatgca tatgatccaa tatcaaagga
      361 aatgatagca ttgaaggatg agactaatcc aattgaggag tggcagcata tagaacagct
      421 aaagggtagt gctgaaggaa gcatacgata ccccgcatgg aatgggataa tatcacagga
      481 ggtactagac tacctttcat cctacataaa tagacgcata taagtacgca tttaagcata
      541 aacacgcact atgccgttct tctcatgtat atatatatac aggcaacacg cagatatagg
      601 tgcgacgtga acagtgagct gtatgtgcgc agctcgcgtt gcattttcgg aagcgctcgt
      661 tttcggaaac gctttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac
      721 ttcagagcgc ttttgaaaac caaaagcgct ctgaagacgc actttcaaaa aaccaaaaac
      781 gcaccggact gtaacgagct actaaaatat tgcgaatacc gcttccacaa acattgctca
      841 aaagtatctc tttgctatat atctctgtgc tatatcccta tataacctac ccatccacct
      901 ttcgctcctt gaacttgcat ctaaactcga cctctacatt ttttatgttt atctctagta
      961 ttactcttta gacaaaaaaa ttgtagtaag aactattcat agagtgaatc gaaaacaata
     1021 cgaaaatgta aacatttcct atacgtagta tatagagaca aaatagaaga aaccgttcat
     1081 aattttctga ccaatgaaga atcatcaacg ctatcacttt ctgttcacaa agtatgcgca
     1141 atccacatcg gtatagaata taatcgggga tgcctttatc ttgaaaaaat gcacccgcag
     1201 cttcgctagt aatcagtaaa cgcgggaagt ggagtcaggc tttttttatg gaagagaaaa
     1261 tagacaccaa agtagccttc ttctaacctt aacggaccta cagtgcaaaa agttatcaag
     1321 agactgcatt atagagcgca caaaggagaa aaaaagtaat ctaagatgct ttgttagaaa
     1381 aatagcgctc tcgggatgca tttttgtaga acaaaaaaga agtatagatt ctttgttggt
     1441 aaaatagcgc tctcgcgttg catttctgtt ctgtaaaaat gcagctcaga ttctttgttt
     1501 gaaaaattag cgctctcgcg ttgcattttt gttttacaaa aatgaagcac agattcttcg
     1561 ttggtaaaat agcgctttcg cgttgcattt ctgttctgta aaaatgcagc tcagattctt
     1621 tgtttgaaaa attagcgctc tcgcgttgca tttttgttct acaaaatgaa gcacagatgc
     1681 ttcgttaaca aagatatgct attgaagtgc aagatggaaa cgcagaaaat gaaccgggga
     1741 tgcgacgtgc aagattacct atgcaataga tgcaatagtt tctccaggaa ccgaaataca
     1801 tacattgtct tccgtaaagc gctagactat atattattat acaggttcaa atatactatc
     1861 tgtttcaggg aaaactccca ggttcggatg ttcaaaattc aatgatgggt aacaagtacg
     1921 atcgtaaatc tgtaaaacag tttgtcggat attaggctgt atctcctcaa agcgtattcg
     1981 aatatcattg agaagctgca gcgtcacatc ggataataat gatggcagcc attgtagaag
     2041 tgccttttgc atttctagtc tctttctcgg tctagctagt tttactacat cgcgaagata
     2101 gaatcttaga tcacactgcc tttgctgagc tggatcaata gagtaacaaa agagtggtaa
     2161 ggcctcgtta aaggacaagg acctgagcgg aagtgtatcg tacagtagac ggagtatact
     2221 agtatagtct atagtccgtg gaattctcat gtttgacagc ttatcatcga taagcttttc
     2281 aattcaattc atcatttttt ttttattctt ttttttgatt tcggtttctt tgaaattttt
     2341 ttgattcggt aatctccgaa cagaaggaag aacgaaggaa ggagcacaga cttagattgg
     2401 tatatatacg catatgtagt gttgaagaaa catgaaattg cccagtattc ttaacccaac
     2461 tgcacagaac aaaaacctgc aggaaacgaa gataaatcat gtcgaaagct acatataagg
     2521 aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc atgcacgaaa
     2581 agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta ctggagttag
     2641 ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc ttgactgatt
     2701 tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac aattttttac
     2761 tcttcgaaga cagaaaattt gctgacattg gtaatacagt caaattgcag tactctgcgg
     2821 gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg gtgggcccag
     2881 gtattgttag cggtttgaag caggcggcag aagaagtaac aaaggaacct agaggccttt
     2941 tgatgttagc agaattgtca tgcaagggct ccctatctac tggagaatat actaagggta
     3001 ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct caaagagaca
     3061 tgggtggaag agatgaaggt tacgattggt tgattatgac acccggtgtg ggtttagatg
     3121 acaagggaga cgcattgggt caacagtata gaaccgtgga tgatgtggtc tctacaggat
     3181 ctgacattat tattgttgga agaggactat ttgcaaaggg aagggatgct aaggtagagg
     3241 gtgaacgtta cagaaaagca ggctgggaag catatttgag aagatgcggc cagcaaaact
     3301 aaaaaactgt attataagta aatgcatgta tactaaactc acaaattaga gcttcaattt
     3361 aattatatca gttattaccc gggaatctcg gtcgtaatga tttttataat gacgaaaaaa
     3421 aaaaaattgg aaagaaaaag ctttaatgcg gtagtttatc acagttaaat tgctaacgca
     3481 gtcaggcacc gtgtatagcg taagaacact agcgactttt gttcgtgaac caacagagta
     3541 ggatttctac tgctacatct cttaggtagt tggttagtcc gatcgctcac ttttggttgt
     3601 tgttaagtac ttcataagtt tatccttttc ctttttcaca ctgagctact ttgggtatag
     3661 cttttggccc aaggatcttt gaattttctc caaaagtact ttattttata tcctacaggt
     3721 tgcggttttc atattttaaa aagcttttaa tcattccttt gcgtatgaag cccaaagtcg
     3781 tcctcaccca ctgggtgcac ccggaaatca tcgaattgtt gtccgctagc gccgatgtta
     3841 tccccaacac cacaagagaa accttgccgc gttctgaggt aattgctaga gccaaagatg
     3901 ctgatgcact catggctttc atgccggaca gcatcgacag cgcttttctc gaggaatgtc
     3961 caaagctgcg tgtcatcggc gccgctctta aaggctatga taacttcgat gtcaacgcct
     4021 gcacaagaca cggtgtatgg cttacgattg tgccggattt gcttacgatc ccgaccgctg
     4081 aactgactat cggccttctt ctcggtttga caaggcatat gctggaaggc gataggcaaa
     4141 tccgtagcgg acacttccaa ggctggagac cgacactata tggctctggt ttgacaggaa
     4201 aaacgcttgg catcattggt atgggggctg tcggccgtgc aatcgcccag agattggctg
     4261 gctttgaaat gaatctcttg tattgcgatc cgattccgct caatgccgaa caagaaaagg
     4321 cttggcacgt acagagagtc acgctcgatg aactgctcga aaaatgtgat tatgtcgtgc
     4381 cgatggttcc gatggccgca gagacactgc atctgatcga tgccaccgct ttggccaaga
     4441 tgaaaaccgg tagctacctg atcaatgcat gtagaggctc ggtcgtggat gagaatgctg
     4501 tgatagcagc actggcttct ggaaaactag ctggatatgc agccgatgtc ttcgagatgg
     4561 aagaatggat aagagctgat agaccgcagg ctatccccaa ggctctgctc gacaatacgg
     4621 cacaaacgtt ttttacgccg catttgggat cggctgtcaa ggaagttaga cttgaaatcg
     4681 agagacaggc agctatgaac atcatccagg cactcgctgg tgaaaaaccg atgggcgcta
     4741 ttaatcagcc gtatccggga gtaaaggctg cttgagagta ttttatctgc aattacggat
     4801 acggttggtc ttatgtagat acatataaat atatatcttt ttctttcttt ttcttagtca
     4861 ggattgtcgt ttagcataat atacatgtag tttatttaat cacataccac tgattatctt
     4921 tagaatttta taaatttttg aaataaatgg gtggctttta atggtgtcta tgttaagtga
     4981 ggcttttaga atgctcttcc tgctttgttt attatatgtg tatgaaagat atgtatgtat
     5041 ttacatgtgt ttgtagcgtc cccagtcaaa acctgtgcgc tatacctaaa tggattgata
     5101 atcttcattc actaattcta aaatagactt cttccccaaa gaacggtgta acgatgaggc
     5161 tctatccagc tgcttatcta aatcaacttt aacgatggat gatcttatga cacggggatc
     5221 tttctttaaa gttcttagaa tttcagactg taccgcagct gatgaatcaa acagcattaa
     5281 aaagtgatat gctcgaaaat gtttttcctg gtctttcttc attattttag gaagatacct
     5341 tatgcccatg ggtacaatgt ccctcaccac acctctgttt tgaataatca gtttcccgat
     5401 tgtggaagac aattcttttg cttccaactt tggcgcattg gagttggtta tgcgaacaag
     5461 tccgatcagc tcataaagca tcttagtgaa aagggtggtt ttgcgttatt ctatggaagc
     5521 cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca attcttgcgg
     5581 agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc cgccatctcc
     5641 agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc
     5701 gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat
     5761 gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga
     5821 gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca
     5881 gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga
     5941 acacctacat ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc
     6001 cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca
     6061 tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg
     6121 aacagaaatt cccccttaca cggaggcatc aagtgaccaa acaggaaaaa accgccctta
     6181 acatggcccg ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg
     6241 acgcggatga acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc
     6301 gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     6361 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     6421 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
     6481 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     6541 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
     6601 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6661 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6721 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6781 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6841 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6901 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6961 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7021 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7081 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7141 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7201 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7261 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7321 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7381 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7441 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     7501 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     7561 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     7621 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     7681 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     7741 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     7801 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt
     7861 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     7921 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7981 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     8041 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8101 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac
     8161 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     8221 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     8281 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8341 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     8401 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     8461 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     8521 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     8581 gccctttcgt cttcaa
//
